Art Original

Identifikasi Tanaman Kelapa Sawit F1 (Elaeis Guineensis Jacq Var. Icalix) Varietas Icalix Menggunakan Penanda Rapd dan Sekuens Trnl-trnl-trnf Intergenic Spacer

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The iCalix oil palm variety is a hybrid resulting from the cross between selected Dura Banting from Malaysia and Pisifera AVROS from Africa. The aim of this study is to identify oil palm plants in the community oil palm plantation in Siabu Village, Salo Subdistrict, Kampar Regency (IC) that are compatible with the Dura Banting parent (D) and the iCalix variety (ICM and ICMA) planted by a private company in Riau Province, using RAPD molecular markers and DNA barcoding through sequencing of the trnL-trnL-trnF Intergenic Spacer (IGS). The research activities include the collection of young oil palm leaf samples from several locations, total DNA isolation, total DNA electrophoresis, total DNA amplification using RAPD and IGS primers, sequencing, and data analysis. The results show that 5 selected RAPD primers produced 59 detected loci, consisting of 53 polymorphic loci (89.83%) and 6 monomorphic loci (10.17%). The UPGMA dendrogram results show that iCalix oil palms in community plantations (IC1-IC10) form a group with iCalix oil palms in the company's block II plantation (ICMA10). Additionally, iCalix oil palms in the company's Block I plantation (ICM1 and ICM2) form a group with the Dura Banting parent oil palms in the company plantation (D1 and D3). IGS sequencing performed on 3 oil palm DNA samples resulted in a consensus sequence of 916 bp. BLAST analysis showed a similarity percentage ranging from 98.14% to 99.24%, an e-value of 0, and a query cover value of 100%. The reference sequence with the highest similarity percentage is Elaeis guineensis (99.24%), while the lowest is Euterpe edulis (98.14%). Phylogenetic analysis results show that the three studied samples form the same group as the species Elaeis guineensis, and based on genetic distance matrix analysis, the three studied samples have a genetic distance of 0.000 with the species Elaeis guineensis, thus it can be concluded that the three studied samples are species of Elaeis guineensis.

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Detail Information

Call Number

Agronomi 633.5 Feg i

Language

Indonesia

NPM

224121003

Publisher

Argonomi : Universitas Islam Riau., 2024

Keyword(s)

iCalix Oil Palm
RAPD
trnL-trnL-trnF Intergenic Spacer Sequence

Other Information

Petugas

Uthi Kurnia

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